FIG. 3.

(Bottom). Colocalization of incorporated BrUTP or dsRNA with P131 or P191 in BY-2 protoplasts. PCV-infected protoplasts were processed for double-label immunofluorescence. P131 (A and D) and P191 (G and J) were detected by indirect immunofluorescence (in green) with purified rabbit antibodies raised against P131 or P191. The distribution of BrUTP (B and H) at 24 hpi and of dsRNA (E and K) at 48 h pi was imaged in the same 0.45-μm optical section as for P131 and P191 and is shown in red. The right column (C, F, I, and L) shows digital superimposition of the two panels to the left. Bar, 10 μm.