FIG. 4.

(Left). Localization of P131 and P191 in transgenic BY-2 protoplasts. Healthy (A and B) or PCV-infected (C to H) ER-GFP protoplasts expressing a GFP targeted to the ER were processed for fluorescence with primary antiserum against P131 at 24 hpi (B and D) or against P191 at 48 hpi (G). Similarly, healthy (I and J) or infected (K to P) Man1::GFP protoplasts expressing a GFP targeted to the Golgi apparatus were processed to visualize the P131 (J and L) or the P191 (O) accumulation sites. The digital superimposition of both fluorescent signals is shown in panels E, H, M, and P. Bar, 10 μm.