FIG. 2.

Detection of individual molecular clones by QHDA. (A) Heteroduplexes formed by probe JC6 with clones (JC13, JC2, and JCI-3C in lanes 2, 3, and 4, respectively) with known percentages of mismatch and different numbers of nucleotide insertions. Lanes 5 to 8 show heteroduplexes between JC6 and mixtures of 2 or 3 of the other clones. (B) Semiquantitative detection of two cloned sequences (clones JC2 and JCI-3C) in mixtures with defined proportions of each clone. The percentages of the two clones within each sample before QHDA-PCR are shown above lanes 1 through 7.