FIG. 2.

Calcium concentrations above 50 μM prevent heat-induced uncoating of rotavirus strain RF in media of low ionic strength. Analysis was by agar electrophoresis. TLPs were suspended in the standard buffer supplemented with 500 mM sorbitol and the indicated nominal calcium concentrations. Before analysis by agar gel electrophoresis at room temperature, the particles were incubated at 37°C for 5 min and then heated for an additional 90 s at 60°C (lanes b to g) or not heated (lanes a and h) as shown. TLPs and DLPs were at 1.9 and 2.4 μg of protein per well, respectively.