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Effect of the calcium present in the membrane vesicle incubation medium on time-dependent rotavirus-induced CF release. CF-loaded vesicles were incubated at 37°C in the membrane buffer supplemented with 200 mM Tris-Cl in the absence (none) or the presence of micromolar concentrations of extra CaCl2, as indicated. Purified TLPs, RF strain, kept at 4°C were added at time zero. The final vesicular and viral protein concentrations were 6 and 13 μg/ml, respectively.
