FIG. 6.

Effect of the ionic strength of the incubation medium on CF release induced by either intact or predecapsidated TLPs. CF-loaded vesicles were incubated at 37°C in the membrane buffer supplemented with either 500 mM sorbitol (curves 3 and 4) or 100 mM sorbitol plus 200 mM Tris-Cl (curves 1 and 2). At time zero, TLPs, strain RF, were added either directly (curves 2 and 4) or after treatment with 10 mM EGTA (curves 1 and 3). The final vesicular and viral protein concentrations were 8.5 and 18.8 μg/ml, respectively.