FIG. 4.

SDS-PAGE and immunoblot analysis of purified NS2. Samples (50 μl) were taken from cell lysate, from purified fractions after anion-exchange chromatography, or from fractions after affinity chromatography with glutathione-Sepharose 4B. Ten microliters of each sample was loaded per well of a 10% gel. The gels were stained with Coomassie blue (A) or blotted to nitrocellulose membranes (B) and probed with an oligoclonal antibody generated from NS2 peptide 67. The primary antibody was used at a 1:1,000 dilution, and the secondary antibody (conjugated to AP) was used at 1:5,000.