FIG. 6.

Immunoblot analysis of purified intact and truncated NS2 proteins. Nitrocellulose membrane blots, corresponding to the SDS-PAGE shown in Fig. 5, were probed with antibodies against NS2. Oligoclonal antibodies generated by peptide 67 (A) or peptide 68 (B) were used at a 1:1,000 dilution. A mixture of monoclonal antibodies (C) was used at a dilution of 1:5,000 for each (B87.29.7, B87.29.4, F13.20.2, and F12.17.2, all reacting against unknown epitopes within NS2). Appropriate secondary antibodies (anti-rabbit or anti-mouse), conjugated to AP, were used at a 1:5,000 dilution.