TABLE 1.

Predicted sizes of each NS2 deletion mutant and corresponding protein^a

Clone	Insert size (bp)	ORF	No. of amino acids	Protein size (kDa)	GST fusion protein size (kDa)
pGEX2T-B17S2	1,123	1062	354	41	67
2T-S2ΔN	904	843	281	32	58
2T-S2Δ67	1,081	1020	340	39	65
2T-S2Δ68	955	894	298	34	60
2T-S2Δ67/68	913	874	291	33	59
2T-S2Δ67/N	862	822	274	32	58
2T-S2Δ68/N	736	696	232	27	53
2T-S2Δ67/68/N	694	654	218	25	51

^aEach insert sequence for the S2 deletion mutants was entered into the SwissProt Tools at ExPaSy (Expert Protein Analysis System; www.expasy.ch). The software was then able to predict the number of amino acids and the size of each truncated protein from the ORF of each deletion mutant. The size of each GST fusion protein was also predicted (GST alone is 26 kDa).