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Subcellular localization of VP22 by indirect immunofluorescence. PK15 cells grown on cover slips were mock infected (A) or infected at an MOI of 10 with PRV Becker (B and C) for 6 h prior to fixation. Cells were stained with rabbit polyclonal antiserum to VP22 and detected with an Alexa-488 secondary antibody. Magnifications: ×120 (A and B) and ×160 (C).
