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Guide RNA sequences used in the CRISPRi knock-down viruses.
| Target | sgRNA sequence (5’ ->3’) |
|---|---|
| Klf9 | sg1: GGCGGGAAGAAGTGTCTCGTsg2: GGAGCTGGAGAATGCCGGAG |
| Klf13 | sg1: GCGCCTGCCACAATGCACGGsg2: GGTCGAGCGGAGGCGACGTT |
| Klf6 |
sg1:
GCTGCGGGAGGGTCCGGACC sg2: GGGCAGCGGAGCTTTGAATA |
| Klf7 |
sg1:
GACAGCGCTGCAGATATAGA sg2: GGAGTGATTAGAGCCCTGGA* |
| Scramble | sg1: CACTACCAGAGCTAACTCAT |
CRISPick sgRNA.
