Table 3. sgRNA Golden Gate primers.
Primers used for Golden Gate cloning into the Lenti-CRISPR-v2 vector.
| Name | Primer sequence (5’ ->3’) |
|---|---|
| GG1 |
F:
TAGCTCTTCACGTGACCGAGGGCCTATTTCCCATGATTCC R: TAGCTCTTCATAGGGCGAATTCAAAAAAGCACCGACTCGG |
| GG2 |
F:
TAGCTCTTCACTAGACCGAGGGCCTATTTCCCATGATTCC R: TAGCTCTTCATGAGGCGAATTCAAAAAAGCACCGACTCGG |
| GG3 |
F:
TAGCTCTTCATCAGACCGAGGGCCTATTTCCCATGATTCC R: TAGCTCTTCAATCGGCGAATTCAAAAAAGCACCGACTCGG |
| GG4 |
F:
TAGCTCTTCAGATGACCGAGGGCCTATTTCCCATGATTCC R: TAGCTCTTCAGCAGGCGAATTCAAAAAAGCACCGACTCGG |
| GG5 |
F:
TAGCTCTTCATGCGACCGAGGGCCTATTTCCCATGATTCC R: TAGCTCTTCACAGGGCGAATTCAAAAAAGCACCGACTCGG |
| GG6 |
F:
TAGCTCTTCACTGGACCGAGGGCCTATTTCCCATGATTCC R: TAGCTCTTCACATGGCGAATTCAAAAAAGCACCGACTCGG |