Table 2.
Primers’ sequences that are employed for quantitative reverse transcription polymerase chain reaction assays.
| Specificity/Target gene | Primer sequence (5′-3′) | Accession No./Ref |
|---|---|---|
| Fatty acids transportation and metabolism-related genes | ||
| SLC27A4 | F- TTCTACCTGGGATCAGGGGG R- AAGTGCAGACTCAGGGCTTC |
XM_015279553.1 |
| PPARα | F- GAATCACCCAGTGGAGCAGAAAA R- TGGCAGCAGTGGAAGATTCG |
AF163809.1 |
| PPARγ | F-CTCCGGCTGAGACTTGACAG R-AATTAACGGGCCAAAACGGC |
NM_001001460.2 |
| ACSL1 | F- AGCCTGGGAGCATTTGTGTT R- CCCAGTGCTGGAACCTGAAT |
XM_015276227.4 |
| CD36 | F: AATGACACCATCACGTGCCT R: CGAGGAACAGCACAGAACCT |
XM_015301527.4 |
| NPC1L1 | F- CAACTTCGCCATCACTGTGC R- ACTGCGGTGTGTCCTCAAAG |
XM_040657257.2 |
| MGAT2 | F- TCTTTCACACGGGAGACTGC R- ATCGTCTCGGGGAACAGGTA |
XM_015276736.4 |
| MTTP | F- CACGTACGGCCTAGACATCC R- CTGTGAAAACTGCACCGTGG |
NM_001109784.3 |
| APOB | F- TCTACTCTCCAGGTCCCAGC R- AACGTCAGCAAATGTTGGGC |
NM_001044633.2 |
| FABP | F- CTTTGCCCCCAGACTCTCAC R- ACCCATACCACCTACGACCA |
AY648562.1 |
| Bacterial counting genes | ||
| Bacteroidetes | F: GGARCATGTGGTTTAATTCGATGAT R: AGCTGACGACAACCATGCAG |
(86) |
| Bifidobacterium species | F: TCGCGTCYGGTGTGAAAG R: CCACATCCAGCRTCCAC |
(87) |
| Firmicutes | F: GGAGYATGTGGTTTAATTCGAAGCA R: AGCTGACGACAACCATGCAC |
(86) |
| Bacillus spp. | F: GGGAAACCGGGGCTAATACCG R: CTTCCTCCGTTTTGTCAACAC |
(88) |
| Enterobacteriaceae | F: CATTGACGTTACCCGCAGAAGAAGC R: CTCTACGAGACTCAAGCTTGC |
(89) |
| Lactobacillus species | F: AGCAGTAGGGAATCTTCCA R: CACCGCTACACATGGAG |
(90) |
| House keeping | ||
| GAPDH | F-GGTGGTGCTAAGCGTGTTA R-CCCTCCACAATGCCAA |
NM205518 |
| TBP | F-GTCCACGGTGAATCTTGGTT R-GCGCAGTAGTACGTGGTTCTC |
Acc:8484 |
| rpoD | F-ACATCGCTAAACGTATCGAA R-GTACTGTTCCAGCAGATAGG |
(91) |
SLC27A4, Solute carrier family 27 member 4; PPARα, PPARγ, peroxisome proliferator-activated receptors alpha and gamma; ACSL1, long-chain acyl-CoA synthetase family member 1; CD36, cluster of differentiation 36; NPC1L1, Niemann-Pick C1-Like 1; MGAT2, monoacylglycerol acyltransferase 2; MTTP, microsomal triglyceride transfer protein; APOB, apolipoprotein B; FABP, fatty acid binding protein; GAPDH, Glyceraldehyde-3-phosphate dehydrogenase; TBP, TATA-binding protein; rpoD, RNA polymerase sigma factor.