TABLE 1.
Detection of viremia (PCV2 DNA) by PCR in sera of inoculated and control pigs
| Group | Inoculum | Route of inoculation | DPI
|
Total no. positive/no. tested | |||||
|---|---|---|---|---|---|---|---|---|---|
| 0 | 7 | 14 | 21 | 28 | 35 | ||||
| 1 | None | 0/10a | 0/10 | 0/10 | 0/10 | 0/5 | 0/5 | 0/10 | |
| 2 | PCV2 live virusb | Intranasal | 0/10 | 0/10 | 7/10 | 5/10 | 0/5 | 0/5 | 8/10 |
| 3 | PCV2 DNAc | Intrahepatic | 0/10 | 0/10 | 8/10 | 6/10 | 3/5 | 3/5 | 9/10 |
| 4 | PCV2 DNAc | Intralymphoid | 0/10 | 0/10 | 2/10 | 8/10 | 2/5 | 0/5 | 8/10 |
a
Ten pigs in each group; number positive/number tested.
b
A biologically pure and homogeneous PCV2 virus stock generated by transfection of PK-15 cells with the PCV2 molecular DNA clone.
c
Cloned PCV2 genomic DNA in pSK plasmid.