TABLE 2.
Seroconversion to PCV2 specific antibodies in pigs inoculated with PCV2 live virus or directly injected with cloned PCV2 plasmid DNA
| Group | Inoculum | Route of inoculation | DPI
|
|||||
|---|---|---|---|---|---|---|---|---|
| 0 | 7 | 14 | 21 | 28 | 35 | |||
| 1 | None | 2/10a | 0/10 | 0/10 | 0/10 | 0/5 | 0/5 | |
| 2 | PCV2 live virusb | Intranasal | 0/10 | 0/10 | 0/10 | 1/10 | 1/5 | 4/5 |
| 3 | PCV2 DNAc | Intrahepatic | 0/10 | 0/10 | 0/10 | 0/10 | 1/5 | 5/5 |
| 4 | PCV2 DNAc | Intralymphoid | 0/10 | 0/10 | 0/10 | 0/10 | 1/5 | 3/5 |
a
PCV2 antibody was measured with an enzyme-linked immunosorbent assay; number positive/number tested.
b
A biologically pure and homogeneous PCV2 virus stock generated by transfection of PK-15 cells with PCV2 molecular DNA clone.
c
Cloned PCV2 genomic DNA in pSK plasmid.