FIG. 6.
Effect of UL13 protein kinase expression on accumulation of gD expressed via recombinant baculovirus. Replicate cultures were either mock infected or infected with approximately 10 PFU of Bac-gD alone (top) or approximately 10 PFU each of Bac-gD and Bac-UL13 (bottom) and were cultured in the presence of 10 mM sodium butyrate. Cells were harvested 24 h after baculovirus infection and solubilized in two-dimensional gel lysis solution. Two-dimensional electrophoretic separations were done as described in Materials and Methods. Electrophoretically separated proteins were transferred to nitrocellulose sheets and reacted with mouse monoclonal antibody directed against gD as described in Materials and Methods.