FIG. 5.
Transduction efficiency in CHO mutant and reference cell lines. Cells (cell types are specified in the legend on the right) were transduced with ca. 0.3 TU/cell, as determined by the reference cell lines (HeLa cells for AAV serotypes 1, 2, 3, and 5 and Cos1 cells for AAV4). The transducing titers are given for each serotype in each cell line. The particle numbers used in this experiment (per microliter) were as follows: rAAV1, 1.2 × 108; rAAV2, 5.6 × 108; rAAV3, 9.1 × 108; rAAV4, 2.3 × 108; and rAAV5, 5.4 × 108. Each bar represents the average of three separate infections, with the standard deviation indicated by an error bar.