Skip to main content
. 2026 Sep 8;21(1):101880. doi: 10.1016/j.jcmgh.2026.101880

Figure 3.

Figure 3

The deletion of Bcl-xL induces the inactivation of the Wnt–β-catenin pathway. (A) SOX9 staining in WT mice (representative images for n = 3 mice/group). (B) SOX9 staining in M-A and M-AB mice. The rates of the SOX9+ cells are quantified (n = 3 mice/group). The P value was calculated using a t test. (C) SOX9 (green) and β-catenin (green) staining in M-A mice. The rates of the SOX9+ cells are quantified, comparing non-nuclear and nuclear β-catenin cells (n = 3 mice/group). The P value was calculated using a t test. (D) RNA–in situ hybridization (ISH) using Lgr5 probe in M-A and M-AB mice. The ratio of the Lgr5+ cells are quantified (n = 3 mice/group). The P value was calculated using a t test. (E) GFP (green) and Bcl-xL (red) staining in Mist1-creERT; Apcflox/flox; Lgr5-GFP-DTR mice. The rates of GFP (=LGR5)-positive cells were quantified, comparing Bcl-xL-low and -high expressing cells (n = 3 mice per group). The P value was calculated using a t test. (F) GFP (green) and β-catenin (red) staining in Mist1-creERT; Apcflox/flox; Lgr5-GFP-DTR mice. The rates of GFP (=LGR5)-positive cells were quantified, comparing non-nuclear and nuclear β-catenin cells (n = 3 mice/group). The P value was calculated using a t test. (G) β-catenin (green) and AQP5 (red) staining in M-A and M-AB mice 2 weeks and 1 month after TAM induction. The rates of nuclear β-catenin cells with AQP5 positivity are quantified (n = 3 mice/group). The P value was calculated using a t test. (H) β-catenin (green) and TROP2 (red) staining in M-A and M-AB mice 2 weeks and 1 month after TAM induction. The rates of nuclear β-catenin cells with TROP2 positivity are quantified (n = 3 mice/group). The P value was calculated using a t test. Scale bars, 100 μm. Mean ± standard error of the mean. ∗P < .05. DAPI, 4′,6-diamidino-2-phenylindole.