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. 2026 Sep 8;21(1):101880. doi: 10.1016/j.jcmgh.2026.101880

Figure 6.

Figure 6

Bcl-xL enhances the proliferation in the Wnt-dependent manner in organoid models. (A) Gene construction schema showing KPA and KPAB organoid models. (B) Hematoxylin and eosin staining in KPA and KPAB organoids. (C) RT-PCR of Bcl2l1 (Bcl-xL) in KPA and KPAB organoids (n = 4/group). The P value was calculated using a t test. (D) Western blotting of Bcl-xL and cyclin D1 in KPA and KPAB organoids are shown (n = 2/group). (E) Bcl-xL (green) andβ-catenin (red) staining in KPA and KPAB organoids. The rates of Bcl-xL+ cells and β-catenin–nuclear-translocated cells were quantified (n = 3 organoids/group). The P value was calculated using a t test. (F) RT-PCR of Axin2, Ccnd1, cMyc, and Lgr4 in KPA and KPAB organoids (n = 4/group). The P value was calculated using a t test. (G) The culture of KPA and KPAB organoids in standard medium. The ratios of organoid diameters relative to those on day 1 were quantified (n = 10/group). The P value was calculated using a t test. (H) The culture of KPA and KPAB organoids in medium lacking Wnt3a, R-spondin 3, and growth factors. The ratios of organoid diameters relative to those on day 1 were quantified (n = 10 per group). The P value was calculated using a t test. (I) Treatment with Bcl-xL inhibitor (100 nM and 1000 nM) in KPA and KPAB organoids. The ratios of organoid diameters relative to those on day 1 were quantified (n = 10 per group). The P value was calculated using a t test. (J) GFP (green) and cleaved caspase 3 (red) staining of KPA and KPAB organoids in the growth factor-free condition. The rates of cleaved caspase 3 cells were quantified (n = 10 organoids/group). The P value was calculated using a t test. Scale bars, 100 μm. Mean ± standard error of the mean. ∗P < .05.