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. 2026 Sep 8;21(1):101880. doi: 10.1016/j.jcmgh.2026.101880

Figure 9.

Figure 9

Bcl-xL–mediated suppression of Hnf4a expression promotes activation of the Wnt–β-catenin pathway. (A) RT-PCR of Hnf4a in KPA organoids and KPA organoids overexpressing Hnf4a (n = 3/group). The P value was calculated using a t test. (B) The infection of KPA organoids with Hnf4a-overexpressing adenovirus. The ratios of organoid diameters relative to those on day 1 were quantified (n = 10 per group). The P value was calculated using a t test. (C–F) Bulk RNA sequencing comparing KPA organoids and KPA organoids overexpressing Hnf4a (n = 3/group). (C) PCA plot, (D) Heat map of top 100 differentially expressed genes (DEGs). (E) GSEA with Gene Ontology Biological Process (GOBP) WNT SIGNALING PATHWAY, WP WNT SIGNALING PATHWAY. v2025, and GOBP EPITHELIAL CELL PROLIFERATIOHN gene sets. (F) Heatmap of the WNT-target genes. (G) RT-PCR of Sox9, Axin2, and Fzd7 in KPA organoids and KPA organoids overexpressing Hnf4a (n = 3/group). The P value was calculated using a t test. Mean ± standard error of the mean. ∗P < .05.