Table 2.
Primer Sequences Used for Quantitative PCR
| Targets | Forward or reverse | Sequences |
|---|---|---|
| Lgr5 | Forward | 5'-CCACAGCAACAACATCAGGT-3' |
| Reverse | 5'-AACAAATTGGATGGGGTTGT-3' | |
| Lgr4 | Forward | 5'-CCTTCACCCAAGCACTGGATA-3' |
| Reverse | 5'-AGGTCGTTACCAGCCAGTTG-3' | |
| Axin2 | Forward | 5'-TGCATCTCTCTCTGGAGCTG-3' |
| Reverse | ACTGACCGACGATTCCATGT-3' | |
| Sox9 | Forward | 5'-GAGGCCACGGAACAGACTCA-3' |
| Reverse | 5'-CAGCGCCTTGAAGATAGCATT-3' | |
| Cd44 | Forward | 5'-ACAGTACCTTACCCACCATG-3' |
| Reverse | 5'-GGATGAATCCTCGGAATTAC-3' | |
| Cmyc | Forward | 5'-GAGCTGTTTGAAGGCTGGATTT-3' |
| Reverse | 5'-TCCTGTTGGTGAAGTTCACGTT-3' | |
| Fzd7 | Forward | 5'-TGCAGTCATGGCGTCGCTTCTA-3' |
| Reverse | 5'-TTCGGCCACTGCCTTTGCCTTT-3' | |
| Fzd5 | Forward | 5'-ATCCTCCGAGAGTTCTGTCCTTGG-3' |
| Reverse | 5'-TCGTCTCCTTCTTCCCTTTGCCT-3' | |
| Ccnd1 | Forward | 5'-CGTGGCCTCTAAGATGAAGGA-3' |
| Reverse | 5'-TCGGGCCGGATAGAGTTGT-3' | |
| Mist1 | Forward | 5'-GTTTTCCCAGGAGGTGGTG-3' |
| Reverse | 5'-AAGCTACGTGTCCTTGTCCC-3' | |
| Bcl2l1 | Forward | 5'-GTGGAAAGCGTAGACAAGGA-3' |
| (Bclxl) | Reverse | 5'-GTTCCCGTAGAGATCCACAA-3' |
| Bcl2 | Forward | 5'-ATGGGGTGAACTGGGGGAGGATTG-3' |
| Reverse | 5'-GGCCAGGCTGAGCAGGGTCTTC-3' | |
| Bax | Forward | 5'-TTGCTGATGGCAACTTCAAC-3' |
| Reverse | 5'-GATCAGCTCGGGCACTTTAG-3' | |
| Bak | Forward | 5'-AGCCGGGAATGCCTACGAAC-3' |
| Reverse | 5'-GGCCCAACAGAACCACACCA-3' | |
| Pparg | Forward | 5'-GGAGCCTAAGTTTGAGTTTGCTGTG-3' |
| Reverse | 5'-TGCAGCAGGTTGTCTTGGATG-3' | |
| Gapdh | Forward | 5'-AGAAACCTGCCAAGTATGATGAC-3' |
| Reverse | 5'-TCATTGTCATACCAGGAAATGAG-3' |