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Pathogenic RL-MuLV mixture does not contain SFFV. Protein extracts isolated from spleens of infected and uninfected BALB/cJ mice and cell lines expressing gp55 SFFV were separated on sodium dodecyl sulfate-acrylamide gels, blotted, and probed with goat anti-F-MuLV gp70 polyclonal antibodies. pSFFV/NRK, protein extract from the NRK cells transfected with SFFV; pSFFV(57)/NRK, protein extract from the cells transfected with SFFV and full-length competent ecotropic MuLV.
