TABLE 1.
Sequences of the oligonucleotide primers and probes used in quantitative real-time PCR
| RNA targeta | Sequence (final concn in reaction mixture [μM]) of:
|
||
|---|---|---|---|
| Forward primer | Reverse primer | Probe | |
| mIDO | GAGAA AGCCA AGGAA ATTTT TAAGA G (0.66) | GATAT ATGCG GAGAA CGTGG AAA (0.22) | TGCGT GACTT TGTGG ACCCA GACAC (0.24) |
| rIDO | TGCTT TGACG TCCTG CTGG (0.66) | TTCCT GTGAG CTGGT GGCA (0.22) | ATGCT GCTCA GTTCC CCCAGG GACA (0.24) |
| rIFN-γ | GCAGA TAATG GAACT CTTTT CTTAG ACA (1.00) | AGGAG ACAAT TTGGC TCTGC AT (1.00) | TTTCT GTCAC TCTCC TCTTT CCAAT TCCTC CAA (0.26) |
| rTNF-α | ATCTT CTCGA ACCCC AAGTG A (0.80) | CGGTT CAGCC ACTGG AGCT (0.80) | CCCAT GTTGT AGCAA ACCCT CAAGC TGA (0.40) |
| SIV | GCAGA GGAGG AAATT ACCCA GTAC (0.40) | CAATT TTACC CAGGC ATTTA ATGTT (1.20) | TGTCC ACCTG CCATT AAGCC CGA (0.40) |
| m/r18S | CGGCT ACCAC ATCCA AGGAA (1.20) | GCTGG AATTA CCGCG GCT (0.40) | TGCTG GCACC AGACT TGCCC TC (0.40) |
a
m, mouse; r, rhesus monkey; m/r, mouse and rhesus monkey.