FIG. 4.

Infection of senescent cells enhances HCMV replication. (A) HELF seeded at both low (2 × 10⁴ cells/cm²) and high (10⁵ cells/cm²) densities were serum starved for 72 h and then stained for SA-β-Gal activity. (B) HELF were infected at an MOI of either 0.2 or 2 and, 24 h p.i., immunohistochemically stained for IE antigens with commercial anti-IEA Abs. Magnification, ×20. (C) HELF were infected at an MOI of either 0.2 or 2 and 8 days p.i. viral titers in the culture supernatants were evaluated and virus yields were plotted.