FIG. 8.

EMSA with RTA412. E. coli-derived RTA412 protein (0.1 μg) was incubated with RE I-2B and RE IIC-2 (A), PAN RRE (B), and 57/K8 RRE (C) as probes, respectively. PAN RRE and 57/K8 RRE generated specific shifted bands marked by triangles and diamonds, respectively. A mouse monoclonal anti-T7 tag antibody (αT7; Novagen) caused supershift (shown as filled circles with a line). PI refers to mouse preimmune serum, and stars indicate nonspecific complexes. (D) Competition anaysis with RE I-2B and RE II-C2 was shown. In this case, each cold competitor was added by a 50-fold up to a 200-fold molar excess, as indicated in the panel.