FIG. 4.
Viral yields in infected Vero and V27 cells. (A) Single-cycle yield assays. Replicate cultures of approximately 2 × 106 Vero or V27 cells were infected in duplicate at an MOI of 1 with WT HSV-1 or various ICP27 mutants. After 24 h, the infections were terminated by freezing. Virus was released from the cells by three cycles of freeze-thawing, and the total infectious virus in the lysate was determining by a plaque assay on V27 cells. The gray and hatched bars represent the mean yields in V27 and Vero cells, respectively. Error bars indicate the values of the duplicate samples. (B) Growth curves. Cultures of approximately 2 × 106 Vero cells were infected in duplicate at an MOI of 1 with WT HSV-1 or various ICP27 mutants. Infections were terminated at multiple time points by freezing, and virus yields were determined as for panel A. The values shown represent the mean yields of duplicate infections.