FIG. 8.

Deletion of the acidic region between residues 21 and 63 does not affect viral growth or ICP27 localization. (A) Immunoblot analysis of dAc ICP27. Vero cells were mock infected or infected with the viruses indicated at an MOI of 10. Protein extracts were prepared at 5 hpi, and immunoblotting was carried out with MAb H1113. (B) Plaquing ability of dAc. Stocks of WT HSV-1 or dAc isolates were titrated by plaque assay on Vero cells (gray bars) or V27 cells (hatched bars). (C) Localization of ICP27 in dAc-infected cells. Vero cells were mock infected or infected with the viruses indicated. The cells were fixed at 6 hpi and processed for immunofluorescence with the H1113 MAb.