FIG. 3.
Neonatal and adult T cells undergo similar maturations in cytokine expression patterns. Splenocytes (4 × 106/well) were stimulated with peptide NP118-126 and subjected to ICCS as described in Materials and Methods. Live cells were gated for the expression of CD8 and analyzed for the presence of TNF-α and IFN-γ (x and y axis, respectively). The TNF-α-IFN-γ double-positive cells in each sample are all shown within the upper right quadrant. The percentage in each panel represents the fraction of cells within the upper right quadrant as a function of the sum of the number of cells within the upper left and upper right quadrants. FITC, fluorescein isothiocyanate; PE, phycoerythrin.