FIG. 4.

Antigen-specific neonatal and adult T cells have similar avidities for peptide. Splenocytes from individual BALB/c mice were obtained 14 days postvaccination with pCMVNP or postinfection with LCMV, stimulated for 5.5 h in vitro in the presence of various concentrations of NP118-126, stained for CD8, and then subjected to ICCS for intracellular IFN-γ as described in Materials and Methods. The average responses (±1 standard deviation) of groups of three neonates (Neonates d14), three adults (Adults d14), and one adult infected with LCMV (Adults+LCMV), each measured in triplicate at each peptide concentration, are shown. For each group, the response at each peptide concentration is expressed as the maximal response (measured at 1,000 nM peptide). The half-maximal response is shown as a solid horizontal line.