FIG. 5.

Neonatal and adult T cells rapidly mobilize cytokine production in response to peptide stimulation. Splenocytes from individual neonatal (Neonates+pCMVNP; n = 3) or adult (Adults+pCMVNP; n = 2) BALB/c mice were obtained 14 days after immunization with 100 μg of pCMVNP. Cells from each individual were stimulated in vitro for 0 to 8 h. Cells (4 × 10⁶) were harvested at each time point, stained with anti-CD8 antibody, and subjected to ICCS as described in Materials and Methods. The average response among groups at each time point is expressed as a percentage of the average response (±1 standard deviation) observed among individuals within that group after 8 h of peptide stimulation.