FIG. 6.
Inhibition of huDC-SIGN-dependent HIV-1 capture and transfer by the anti-ptDC-SIGN MAb. THP-1/huDC-SIGN cells were mock treated or incubated with 50 μg of anti-ptDC-SIGN MAb 11C1 (A) or 8C1 (B) per ml prior to incubation with an HIV-luc reporter virus pseudotyped with either the HIV-1ADA or HIV-1JRFL envelope. Following the incubation with virus, cells were washed and cultured with HOS-CD4-CCR5 cells. Viral infection was determined by measuring luciferase activity in the target cells. THP-1/DC-SIGN− cells were used for background virus capture and transfer. Relative luciferase activity ± the standard error of the mean is shown.