FIG. 6.
Growth of virus in TIAR and TIA-1 knockout cell lines. Confluent monolayers of wild-type (CONT) (W4; TIAR+/+ TIA-1+/+), TIA-1 knockout (TIA-1-KO) (a−/−43; TIAR+/+ TIA-1−/−), and TIAR knockout (TIAR-KO) (NaR4; TIAR−/− TIA-1+/+) cells were infected with WNV (A), VSV (C), Sindbis virus (D), HSV-1 (E), or vaccinia virus (F) at an MOI of 1, and confluent monolayers of TIAR-REC or Cont-REC cells were infected with WNV (B) at an MOI of 1. Culture fluid samples were taken at the indicated hours p.i. and titered in duplicate by plaque assay in the cell lines indicated in Materials and Methods. Each growth curve was repeated two or three times, and representative growth curves are shown. All virus titers were expressed as PFU per cell because the various cell lines grew to different but characteristic densities when confluent. All peak virus titers were in the range of 106 to 107 PFU/ml.