Fig. 2.

Δ⁹-THC produces dose-dependent antinociception in the hotplate test. Δ⁹-THC (1, 3, 10, and 30 mg/kg s.c.) was administered twice daily over a period of 5 days (A). Δ⁹-THC produced dose-dependent antinociception in males (B) and females (C) at the 30 minutes timepoint on day 1 of dosing, expressed as percent maximum possible effect (%MPE). Time course of antinociception on day 1 is shown for males (D) and females (E). Tolerance developed by day 3 (males: F, females: G) and remained by day 5 (males: H, females: I) in both sexes. Data are represented as mean ± SEM. n = 6–7 per group. Colored asterisks denote Tukey’s post hoc comparisons against vehicle at the indicated timepoint for the dose of the corresponding color (∗P < .05, ∗∗ P < .01, and ∗∗∗ P < .001). Black symbols denote the treatment × time interaction for that panel (^####P < .0001), two-way repeated-measures ANOVA. Complete pairwise comparisons for every timepoint at which any comparison reached significance are reported in Supplemental Table 2.