FIG. 1.

RT-PCR analysis of IFN-β mRNA in cells infected with HAV. FRhK-4 cells were infected with HAV/7 at an MOI of 4 and MRC-5 cells were infected with HAV-GBM at an MOI of 1. Cells were transfected with 20 μg of poly(IC) [poly(IC)+] as positive controls and with DEAE-dextran without poly(IC) [poly(IC)−] as negative controls. Proof of β-actin mRNA by RT-PCR was included as control to ensure that equal quantities of RNA were analyzed. Shown are the data of an experiment representative for a series of three, each including two replicates.