FIG. 3.

RT-PCR analysis of dsRNA-induced IFN-β mRNA in cells infected with HAV. FRhK-4 cells infected with HAV/7 at an MOI of 3 and MRC-5 cells infected with HAV-GBM at an MOI of 1 (+), as well as mock-infected cells (−) were transfected with 20 μg of poly(IC)/ml at days 1, 4, and 6 p.i., and IFN-β mRNA expression was analyzed 12 to 16 h after transfection. Proof of β-actin mRNA by RT-PCR was included as a control to ensure that equal quantities of RNA were analyzed. Shown are the data of an experiment representative for a series of three, each including two replicates.