FIG. 6.
Influence of HAVcyt/HB1.1 on dsRNA-induced apoptosis. HAVcyt/HB1.1-infected FRhK-4 cells, as well as HAV/7-infected FRhK-4 cells (MOI 4) and mock-infected FRhK-4 cells, were transfected with 20 μg of poly(IC)/ml at 24 h p.i. [+poly(IC)]. The percentage of cells with a DNA content of <2N, which is indicative of apoptosis, was determined by flow cytometry 12 h after treatment with poly(IC). As controls, the cells were mock transfected [−poly(IC)]. Additionally shown is the analysis of mock-transfected cells 14 days p.i. as an example for the effect of the HAVcyt/HB1.1 variant with regard to the induction of apoptosis at a later stage of infection. The data are means obtained from two separate experiments, including two replicates, and error bars indicate the standard deviations.