TABLE 2.
Influence of the HAVcyt/HB1.1 infection on dsRNA-induced CAT expression in FRhK-4/(−110IFN-β)-CAT cells in comparison with mock- and HAV/7-infected cellsa
| Infection (MOI) | Mean CAT concn (pg/ml)b | % Reduction of CAT compared to medium | HAV TCID50/mlb |
|---|---|---|---|
| Medium | 844 (83) | 0 | 0 |
| Cell lysate | 830 (40) | 2 | 0 |
| HAV/7 (0.04) | 535 (36) | 37 | NDc |
| HAV/7 (0.4) | 138 (18) | 84 | 2.2 × 103 (0.6) |
| HAV/7 (4) | 5 (2) | 99 | 3.3 × 105 (0) |
| HAVcyt/HB1.1 (0.04) | 968 (50) | 0 | ND |
| HAVcyt/HB1.1 (0.4) | 614 (40) | 27 | 1.3 × 106 (0.6) |
| HAVcyt/HB1.1 (4) | 142 (12) | 83 | 1.5 × 107 (0.3) |
| HAVcyt/HB1.1 (10) | 68 (9) | 92 | ND |
a
Mock- and HAV-infected cells were transfected with 20 μg of poly(IC)/ml by DEAE-dextran 24 h p.i. and 16 h after induction, CAT expression was analyzed, and the TCID50 titer was determined. The data are means obtained from two separate experiments, including two replicates.
b
The standard deviation is shown in parentheses.
c
ND, not determined.