TABLE 3.
Proof of HAV dsRNA in FRhK-4 cells infected with HAV/7 and HAVcyt/HB1.1 with an MOI of 1 by HDAa
| Time p.i. (h) | HAV/7
|
HAVcyt/HB1.1
|
||
|---|---|---|---|---|
| dsRNAb | CPEc | dsRNAb | CPEc | |
| 0 | − | − | − | − |
| 8 | − | − | − | − |
| 16 | − | − | − | − |
| 24 | − | − | − | − |
| 40 | − | − | + | + |
| 48 | − | − | +++ | + |
| 72 | + | − | ++ | ++ |
a
Additionally, the morphological findings (CPE) indicative for apoptosis are included. Shown are the data of two separate experiments, including two replicates.
b
−, not detected; +, 50 to 500 luminescence counts per second (LCPS)/105; ++, 500 to 1,000 LCPS/105; +++, >1000 LCPS/105.
c
That is, the presence of scattered regions with small, shrunken, and rounded cells. −, no CPE; +, small regions; ++, large regions.