TABLE 1.
Protein | % of wild-type radioactivity
|
||
---|---|---|---|
vLEF12-βgal | vLEF12-ΔATG | Revertant | |
gp64 | 93 ± 4 | 96 ± 3 | 106 ± 6 |
p39/capsid | 46 ± 15 | 52 ± 5 | 89 ± 5 |
pp31 | 107 ± 11 | 86 ± 12 | 97 ± 11 |
p24 | 58 ± 8 | 60 ± 8 | 88 ± 19 |
S. frugiperda cells were separately infected with four different viruses and pulse-labeled with [35S]methionine from 20 to 24 h postinfection. Viral proteins were immunoprecipitated and separated by SDS-polyacrylamide gel electrophoresis. Gels were quantitated by PhosphorImager analysis, using the object average of the uninfected sample to subtract background. Values are expressed as percentages of the wild-type value. The values shown here represent means and standard deviations of triplicate determinations.