Figure 12.

Detection of ADAMTS1 expression in normal kidney and nephroblastoma tissues. RNA samples 3,4, 5 and 20 were prepared from MAV1-induced nephroblastomas collected 18 weeks after injection of MAV1 and RNA samples 8,12, and 12 were prepared from normal kidney of 18 week old chicken. Electrophoresis and Northern blotting were performed as described in the text. The resulting blot was hybridized under stringent conditions with radiolabeled cDNA corresponding to the chicken ADAMTS1 sequence. Samples of glyceraldehyde 3 phosphate dehydrogenase RNA were used as quantitation standard.