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. Author manuscript; available in PMC: 2006 Feb 15.
Published in final edited form as: Proteomics. 2006 Feb;6(4):1250–1260. doi: 10.1002/pmic.200500447

Figure 1.

Figure 1

Increased levels of CYP2E1 and 3-nitrotyrosine in alcohol-fed mouse liver. (A) Equal amounts of microsomal proteins (10 μg/well) from the livers of control mice or alcohol-fed mice for 6 weeks (n = 2 per lane) were separated on 10% SDS-PAGE, transferred to PVDF-Immobilon membranes, and stained with Coomassie blue (top) or subjected to immunoblot analysis by using anti-CYP2E1 antibody (bottom). The CYP2E1 protein band is indicated with an arrow. (B) Equal amounts (15 μg/lane) of freshly isolated cytosolic proteins from the same set of mouse livers were separated on 10% SDS-PAGE and subjected to immunoblot analyses using specific antibodies against 3-nitrotyrosine (3-NT) (top) or β-actin (bottom). Specific protein bands recognized by the antibodies against 3-NT are designated with arrows. This figure represents a typical result from two separate experiments.