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. Author manuscript; available in PMC: 2006 Feb 15.
Published in final edited form as: Proteomics. 2006 Feb;6(4):1250–1260. doi: 10.1002/pmic.200500447

Figure 5.

Figure 5

Inactivation of peroxiredoxin through oxidation after alcohol exposure. (A) Equal amounts (20 μg/well) of cytosolic proteins from pair-fed control and alcohol-fed mice were separated on 12% SDS-polyacrylamide gel and subjected to immunoblot analysis using a specific antibody which recognizes either oxidized Prx-SO3 (inactive form) or Prx (total). (B) Equal amounts (20 μg/well) of cytosolic proteins from E47 HepG2 cells untreated or treated with 100 mM ethanol for indicated times were subjected to immunoblot analysis using specific antibodies to Prx-SO3 or Prx. This figure represents a typical result from three separate experiments.