Table 1.
Summary of LC-MS/MS peptide sequence analyses for protein identification.
| Spot No. | Protein Identified | Pept. ID.ed | Expected Mass/pI | Observed Mass/pI | Accession No. |
|---|---|---|---|---|---|
| 1 | Formyltetrahydrofolate-dehydrogenase* | 2 | 98/5.6 | 84/7.3 | O75891 |
| 2 | ATPase, endoplamic reticulum | 6 | 89/5.1 | 82/7.9 | P55072 |
| 3 | Serum albumin precursor | 8 | 69/5.9 | 82/8.3 | P02768 |
| 4 | Glucose regulated protein precursor 78 kDa | 19 | 72/5.1 | 76/8.2 | P11021 |
| 5 | Glucose regulated protein precursor 75 kDa | 6 | 74/5.9 | 74/7.4 | P38646 |
| 6 | Serotransferrin precursor (Transferrin) | 2 | 77/6.8 | 74/7.5 | P02787 |
| 7 | Heat shock protein 71 | 9 | 71/5.4 | 73/7.7 | P11142 |
| Heat shock protein 70* | 3 | 70/5.6 | 73/7.7 | P54652 | |
| 8 | Serum albumin precursor | 1 | 69/5.9 | 71/8.7 | P02768 |
| 9 | Ubiquilin 1 | 3 | 62/5.0 | 70/8.7 | Q9UMX0 |
| 10 | Alpha-actin 2* | 3 | 42/5.2 | 66/8.0 | P62736 |
| 11 | Ubiquilin 1 | 1 | 62/5.0 | 60/7.5 | Q9UMX0 |
| 12 | Heat shock protein 60 | 2 | 61/5.7 | 60/7.6 | P10809 |
| 13 | Protein disulfide isomerase A3 precursor* | 3 | 57/6.0 | 59/6.6 | P30101 |
| 14 | Catalase* | 1 | 60/6.9 | 59/6.7 | P04040 |
| 15 | Protein disulfide isomerase A3 precursor* | 3 | 57/6.0 | 59/6.9 | P30101 |
| 16 | Catalase* | 1 | 60/6.9 | 59/7.1 | P04040 |
| 17 | Prolyl 4-hydroxylase beta subunit (PDI)* | 3 | 57/4.8 | 59/7.3 | P07237 |
| 18 | Cytokeratin 1 | 3 | 66/8.1 | 59/7.4 | P04264 |
| 19 | Serum Albumin precursor | 1 | 69/5.9 | 58/7.9 | P02768 |
| 20 | Prolyl 4-hydroxylase beta subunit (PDI)* | 1 | 57/4.8 | 58/8.0 | P07237 |
| 21 | Prolyl 4-hydroxylase beta subunit (PDI)* | 15 | 57/4.8 | 58/8.6 | P07237 |
| 22 | Arachidonate 12-lipoxygenase | 1 | 76/5.8 | 58/8.8 | P18054 |
| 23 | Thioredoxin* | 1 | 12/4.8 | 54/6.6 | P10599 |
| 24 | Serum albumin precursor | 1 | 69/5.9 | 55/6.7 | P02768 |
| 25 | Tryptophan/serine protease | 1 | 55/6.9 | Q6UWB4 | |
| 26 | Cytokeratin 10 | 5 | 59/5.1 | 55/7.4 | P13645 |
| 27 | Trypsin I precursor (Trypsinogen) | 1 | 27/6.1 | 55/7.5 | P07477 |
| 28 | Selenium binding protein* | 1 | 52/6.1 | 54/7.4 | Q13228 |
| 29 | UDP-glucose-pyrophosphorylase 1 | 2 | 57/7.7 | 57/7.7 | Q07131 |
| 30 | 26S Protease regulatory subunit 6A | 1 | 49/5.1 | 52/8.2 | P17980 |
| 31 | Dynactin complex 50 kDa subunit | 2 | 44/5.1 | 51/8.0 | Q13561 |
| 32 | Protein disulfide isomerase A6 precursor* | 2 | 48/5.0 | 52/7.1 | Q15084 |
| 33 | Argininosuccinate lyase* | 1 | 52/6.5 | 52/7.3 | P04424 |
| 34 | Cytokeratin 9 | 3 | 61/5.1 | 50/6.8 | P35527 |
| 35 | Cytokeratin 10 | 2 | 59/5.1 | 50.7.1 | P13645 |
| 36 | Cytokeratin 10 | 2 | 59/5.1 | 50/7.3 | P13645 |
| 37 | Cytokeratin 10 | 2 | 59/5.1 | 50/7.6 | P13645 |
| 38 | Methionine S-adenosyltransferase* | 1 | 43/5.9 | 50/8.1 | Q00266 |
| 39 | Alpha-actin 2* | 3 | 42/5.2 | 44/7.3 | P62736 |
| 40 | Alpha-actin 2* | 3 | 42/5.2 | 42/7.9 | P62736 |
| 41 | Beta-actin* | 3 | 42/5.3 | 42/8.0 | P60709 |
| 42 | Cytokeratin 9 | 2 | 62/5.1 | 43/8.1 | P35527 |
| 43 | S-Adenosyl homocysteine hydrolase* | 1 | 48/5.9 | 43/8.3 | P23526 |
| 44 | Vitamin D-binding protein precursor | 1 | 53/5.4 | 41/7.0 | P02774 |
| 45 | Cytokeratins 9 and 10 | 11 | 62/5.1 | 41/7.3 | P35527 |
| 46 | Cytokeratins 1 and 2 | 11 | 66//8.1 | 41.7.6 | P35908 |
| 47 | Calcium binding protein 39-like | 1 | 39/8.5 | 41/7.8 | Q9H9S4 |
| 48 | Serotransferrin precursor (Transferrin) | 1 | 77/6.8 | 37.7.2 | P02787 |
| 49 | Glyceraldehyde-3-phosphate dehydrogenase | 1 | 36/6.6 | 38/7.6 | P00354 |
| 50 | Serotransferrin precursor (Transferrin) | 1 | 77/6.8 | 37/8.3 | P02787 |
| 51 | Serum albumin precursor | 2 | 69/5.9 | 33/8.8 | P02768 |
| 52 | Serum albumin precursor | 3 | 69/5.9 | 31/7.5 | P02768 |
| 53 | Serotransferrin precursor (Transferrin) | 1 | 77/6.8 | 31/7.0 | P02787 |
| 54 | Cytokeratin 1 | 3 | 66/8.1 | 23/8.1 | P04264 |
| 55 | Peroxiredoxin 1* | 2 | 22/8.3 | 22/8.3 | Q13162 |
| 56 | Prolyl 4-hydroxylase beta subunit (PDI)* | 2 | 57/4.8 | 57/4.8 | P07237 |
| 57 | Prolyl 4-hydroxylase beta subunit (PDI)* | 1 | 57/4.8 | 53/5.1 | P07237 |
| 58 | Phosphoglucomutase* | 1 | 61/6.3 | 53/5.0 | P36871 |
| 59 | Catalase* | 1 | 60/6.9 | 53/5.2 | P04040 |
| 60 | Serum albumin precursor | 2 | 69/5.9 | 43/5.0 | P02787 |
| 61 | Catalase* | 1 | 60/6.9 | 43/5.2 | P04040 |
| 62 | Glucose regulated protein precursor 78 kDa | 1 | 72/5.1 | 40/4.6 | P11021 |
| 63 | Prolyl 4-hydroxylase beta subunit (PDI)* | 2 | 57/4.8 | 36/4.7 | P07237 |
| 64 | Prolyl 4-hydroxylase beta subunit (PDI)* | 2 | 57/4.8 | 35/4.8 | P07237 |
| 65 | Cytokeratin 9 | 6 | 62/5.1 | 32/4.7 | P35527 |
| 66 | Cytokeratin 1 | 10 | 66/8.1 | 32/4.9 | P04264 |
| 67 | Prolyl 4-hydroxylase beta subunit (PDI)* | 4 | 57/4.8 | 30/4.5 | P07237 |
| 68 | Glucose regulated protein precursor 78 kDa | 2 | 72/5.1 | 30/5.0 | P11021 |
| 69 | Alpha-actin 2* | 2 | 42/5.2 | 29/5.3 | P62736 |
| 70 | Calcium binding protein 39-like | 1 | 39/8.5 | 29/5.4 | Q9H9S4 |
| 71 | Serum albumin precursor | 3 | 69/5.9 | 27/4.6 | P02768 |
| 72 | Glucose regulated protein precursor 78 kDa | 2 | 72/5.1 | 27/4.8 | P11021 |
| 73 | Alpha-actin 2* | 2 | 42/5.2 | 26.5.2 | P62736 |
| 74 | Formyltetrahydrofolate-dehydrogenase* | 1 | 98/5.63 | 26.5.3 | O75891 |
| 75 | S-Adenosyl homocysteine hydrolase* | 1 | 48/5.9 | 26/5.5 | P23526 |
| 76 | Cytokeratin 14 and 16 | 6 | 52/5.1 | 26/5.1 | P02533 |
| 77 | Peroxiredoxin 1/4* | 2 | 30/5.9 | 25/5.1 | Q13162 |
| 78 | Cytokeratin 10 | 2 | 59/5.1 | 24/5.0 | P13645 |
| 79 | Serum albumin precursor | 5 | 69/5.9 | 21/4.7 | P02768 |
| 80 | Transcription elongation factor B | 1 | 12/4.7 | 21/4.8 | Q15369 |
| 81 | Prolyl 4-hydroxylase beta subunit | 1 | 57/4.8 | 21/4.9 | P07237 |
| 82 | Beta-actin* | 1 | 42/5.3 | 18/4.9 | P60709 |
| 83 | Glucose regulated protein precursor 78 kDa | 3 | 72/5.1 | 17/4.8 | P11021 |
| 84 | Calcium binding protein 39-like | 1 | 39/8.5 | 17/5.1 | Q9H9S4 |
| 85 | Retinol Dehydrogenase 10 (RDH10) | 1 | 12/4.7 | 13/5.0 | Q8IZV5 |
| 86 | Cytokeratins 1 and 10 | 5 | 66/8.1 | 12/4.9 | P04264 |
| 87 | Alpha-actin 2* | 1 | 42/5.2 | 12/4.7 | P62736 |
| 88 | Serum albumin precursor | 1 | 69/5.9 | 12/4.9 | P02768 |
| 89 | Serum albumin precursor | 1 | 69/5.9 | 12/5.1 | P02768 |
| 90 | Cytokeratins 1 and 10 | 8 | 66/8.1 | 12/4.6 | P04264 |
| 91 | Cytokeratin 10 | 1 | 59/5.1 | 11/4.9 | P13645 |
| 92 | Cytokeratin 10 | 1 | 59/5.1 | 11/4.8 | P13645 |
The presence of certain proteins present in small quantities might have been missed due to their very low abundance on the 2-D gels and contamination with other proteins that are present in large quantities. Despite small number of peptides identified for some proteins, we are confident that those peptides that were identified by MS analysis represent the peptide sequences of listed proteins. The presence of the proteins marked with asterisks was also confirmed by MS analysis of Coomassie blue-stained 1-D gel pieces (as Supplemental Table 1), as described in the text.