Figure 3.

FACS analysis of CD11b and PKH26 labeling of AM from NO₂-expsoed C57BL/6 mice. Mice were intravenously given PKH26 in combination with diluent C three days prior to onset of a seven days NO₂-exposure. Afterwards, AM were stained with F4/80-Alexa647 and CD11b-FITC. Isotype control (A), CD11b (B) and PKH26 (C) staining was subsequently analyzed by flow cytometry within the F4/80-positive cell population. (C) The proportion of PKH26-negative cells is shown in blue. Part (D) shows a separate analysis of CD11b-expression in PKH26-negative (blue histogram) and PKH26-positive AM (pink histogram) thereby clearly demonstrating that the CD11b-positive cell population mainly consists of PKH26-negative, newly recruited AM. Shown are representative results of eight animals per group.