Table 2.
Overview of cell surface expression of several cell surface molecules on rat alveolar macrophages and detection of differential expression in AM from NO2-exposed rats in comparison to AM from untreated controls. Expression analysis was performed by flow cytometry following staining of cells with the respective primary antibody and a signal amplification system.
| Antibody | Antigen/Cell population | Expression | Differences |
| 1A29 | ICAM-1 (CD54) | medium | medium |
| 1C7 | mononuclear phagocytes (CD68 ?) | medium | medium |
| 3.2.3. | NKR-P1 (CD161) | weak | no |
| 3A12 | PECAM-1 (CD31) | weak | no |
| 5F10 | VCAM-1 | no | |
| ART18 | IL-2 receptor | no | |
| ART65 | IL-2 receptor | no | |
| ED2 | macrophage subset (no monocytes) | no | |
| ED3 | macrophage subset (no monocytes) | no | |
| ED4 | macrophages | medium | no |
| ED7 | macrophage subset (CD11/CD18; CR3) | medium | strong |
| ED8 | macrophage subset (CD11/CD18; CR3) | medium | small |
| ED9 | macrophage subset (SIRPα, CD172a) | strong | medium |
| KIM2R | mature tissue macrophages | no | |
| MAR3 | macrophage subset | no | |
| Ox2 | CD200 | no | |
| Ox26 | transferrin receptor (CD71) | no | |
| Ox3 | MHC-II (I-A like) | weak | small |
| Ox4 | MHC-II (I-A like) | weak | small |
| Ox41 | macrophages, DCs, PMNs (SIRP) | no | |
| Ox50 | hyaluronic acid receptor (CD44) | medium | small |
| Ox52 | activated monocytes | ||
| Ox6 | MHC-II (RT1.B; I-A) | weak | medium |
| Ox62 | DC subpopulation | no | |
| Ox8 | CD8α | no | |
| Ox85 | L-selectin (CD62L) | no | |
| RM-1 | monocytes/macrophages/DCs/PMNs | strong | small |
| RM-4 | all macrophages (no monocytes) | medium | strong |
| RMA | macrophage subset (120 kDa antigen) | medium | medium |
| RP-1 | neutrophiles (intracellular) | no | |
| RP-3 | neutrophiles (intracellular) | no | |
| W3/13 | leukosialin (CD43) | no | |
| WT/1 | LFA-1 (CD11a) | weak | no |