Table 1.

Primers and PCR conditions used in BACH1 mutation analysis.

Exon	Strand	Primer name	Nucleotide sequence (5'→3')	Amplicon lenght (bp)	Annealing temperature (°C)
2	Forward	Ex2F	CTGTTTCCAGATTTCTCCC
	Reverse	Ex2R	GTGAACCCAGAAAATATTCTCC	331	58
3	Forward	Ex3F	CCCTGGAGTGCAATCTCACT
	Reverse	Ex3RN	TAGCGACAGCATGGCTGAA	319	48
4	Forward	Ex4F	CCTGGGTGAACTGGGCTGTAG
	Reverse	Ex4RN	TAACAGTAATAATTAAGACTC	316	48
5	Forward	Ex5FN	TTGCCTACCTGTAAGTTATTTATG
	Reverse	Ex5RN	ACCATGTTCAGCTGTAACTAACTG	233	60
6	Forward	Ex6F	GAGCTGTTTTGGCCTTTGAGA
	Reverse	Ex6RN	CTGAGTGGGTTGCTACTGTCCT	317	55
7	Forward	Ex7F	GTTCTGATTCCATGTGAGGTT
	Reverse	Ex7R	GTACATATAAAACACATACTGAGT	448	55
8	Forward	Ex8F	GATGTTCCTCAAATTCTGAGATAA
	Reverse	Ex8R	CATCTAAAAGCTTTTACATTCAAC	386	55
9	Forward	Ex9F	GCCTATAGTGTGAATTTTAAAATG
	Reverse	Ex9R	CCTAGTTAACCAAAGTTTACTAAC	395	55
10	Forward	Ex10F	GATCAACGCATGACAATAATGATG
	Reverse	Ex10R	GGGTTACTCACTAGATTTAATCTG	320	55
11	Forward	Ex11F	GCATGTTTTGTTGGGTTTCATTGT
	Reverse	Ex11R	GGTATGTATTAAACACATGCTAGC	326	55
12	Forward	Ex12F	GTACCAGCTCTTTCAAATGAG
	Reverse	Ex12R	CTATCTTTAAAAGAGTCAACCAC	360	55
13	Forward	Ex13FN	GTGCTGGGATTACAGGTGTGAGCCA
	Reverse	Ex13RN	ACTTGCTGGCACTTCAGGTATCTTC	317	60
14	Forward	Ex14F	CTTGTTGCTTGATCTTTTATGTAC
	Reverse	Ex14R	CTAGGAAGCTTACTGTGGTAA	356	55
15	Forward	Ex15FN	ACAGCTCTATGAGATATATTG
	Reverse	Ex15RN	TCATAGGAGAACAAGTACAAT	504	55
16	Forward	Ex16FN	TTACTTAAAGACATTGAACTT
	Reverse	Ex16RNN	CACTATAAAAGCAAAGCGC	392	54
17	Forward	Ex17FN	CTGTTAGAAGTTAATATGATG
	Reverse	Ex17RN	GAATACATACCAGTTCCTATG	325	55
18	Forward	Ex18F	CCAATTTTCTGTCTGTCCCAC
	Reverse	Ex18R	GATAGTAGAGCTCATGTTATGTG	254	55
19	Forward	Ex19F	CTTCACTAGAAAAAGCAAGTG
	Reverse	Ex19R	CCACCATATTTAAGGAATTAATC	523	48
20A	Forward	Ex20AFN	ACCTAGCAATTATGTTAGCT
	Reverse	Ex20ARN	TCTGTATCTTCAGGATCGTA	584	48
20B	Forward	Ex20BFN	ATTGATGCCACCCTTACTA
	Reverse	Ex20BRNN	TAACATAAGCATGATGACATA	565	54