Table 1.
Doubling time for A549 and A549/p21 clones.
| Doubling time (hours)1 | ||
| Sample | w/ | w/o |
| antibiotic2 | antibiotic | |
| A549 (parental) | NA3 | 22.3 |
| A549/p21 clone 1 | 39.3 | 38.5 |
| clone 29 | 39.5 | 38.2 |
| clone 30 | 39.3 | 39.3 |
| clone 32 | 38.2 | 37.7 |
1) Doubling time was calculated by plotting the graph of cell count vs. time using the third degree polynomial. 2) Populations grown in 450 μg/mL G418 (minimum lethal dose 100). 3) No growth of parental A549 in medium containing selectable antibiotic; all populations grown in RPMI and 10% FBS.