Skip to main content
. 2006 Feb 15;20(4):417–422. doi: 10.1101/gad.374406

Figure 3.

Figure 3.

Elevated insulin and circulating sugar in miR-278 mutants. (A,B) Q-PCR on RNA from adult flies (A) or adult heads (B) normalized to rp49 RNA. Animals were grown under controlled conditions. AKH RNA was unaltered, whereas the Drosophila insulin-like peptides ILP2, ILP3, and ILP5 were upregulated. (C) Total body triglycerides normalized to total body protein for flies of the indicated genotypes. (D) Survival rates. Fifty first instar larvae were seeded per tube and eclosing adult flies counted. Samples indicate the average of 10 tubes. The reduced survival of the chico mutant was significantly rescued by reduction of miR-278 (t-test = 1.2 × 10–7). Error bars indicate standard deviation. (E) Ratio of glucose and trehalose levels in adult males grown under controlled conditions (Teleman et al. 2005b). Trehalase was omitted for the glucose measurement. (F) Expression level of 4E-BP in control and mutant tissues by Q-PCR. (G) Insulin-induced changes in gene expression in wild-type and mutant larvae by Q-PCR. Values show 4E-BP levels 1 h post-insulin injection as percentage of mock injection.