Figure 3.

The TPR subunits form a stable subcomplex that includes Cdc27, Cdc16, Cdc23, and Cdc26. (A) SDS-PAGE gel and immunoblot of a Cdc23-TAP purification from an apc1Δ strain. Loading is as in Figure 1B. The “Apc⁺” lane is the “eluate 2” fraction from a Cdc23-TAP Apc⁺ purification. (B) Cdc23-TAP purifications from Apc⁺ and apc1Δ strains were normalized by Cdc23-TAP levels and blotted with antibodies against Cdc26. (C) Cdc23-TAP purifications as in B were resolved by SDS-PAGE and visualized by silver staining. (D,E) Small-scale TAP purifications from untagged, CDC23-TA Apc⁺, or CDC23-TAP apc1Δ strains carrying either APC4-3Flag (D) or APC5-3Flag (E). Two independent purifications are shown for each.