Figure 7.
The IR domain of Cdc20 is dispensable for viability. (A) cdc20Δ GAL-CDC20 strains transformed with empty vector or 2 μ vectors carrying CDC20, cdc20-R145D, or cdc20-R610D were streaked on YEP + dextrose and YEP + galactose plates. (B) Ten tetrads dissected from sporulated cdc20-ΔIR/CDC20 heterozygous diploids. (C) SDS-PAGE gel and immunoblot of two tetrads pictured in A, blotted with an N-terminal Cdc20 antibody. (D) SDS-PAGE and immunoblot of strains with combinations of cdc27Δ, cdh1Δ, and cdc20-ΔIR alleles. Two independent isolates are shown in each case. Apc+ and apc11Δ strains are included as controls, both of which are also pds1Δ clb5Δ SIC110X. In both C and D, equivalent amounts of log-phase, asynchronous cultures were used, and the asterisk denotes a background band that serves as a loading control.